Identification of Bx02 Alleles and Genetic Analysis of the Involved Ancestry / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1749-1752, 2015.
Article
in Zh
| WPRIM
| ID: wpr-272528
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the genotypes of the blood sample whose blood grouping showed discrepancies and study the ABO alleles' molecular characteristics of the involved ancestry.</p><p><b>METHODS</b>Blood samples were preliminary genotyped by PCR-SSP. Complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and cloning sequenced to identify its genotype.</p><p><b>RESULTS</b>Sequence analysis indicated that 3 samples of the family had an nt905A>G mutation in the B gene compared with ABO*B101. Combined with the serological results, the propositus could be typed as Bx02/O102.</p><p><b>CONCLUSION</b>DNA sequencing analysis is able to identify the serological phenotype samples that forward and reverse group methods were incongruous.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phenotype
/
ABO Blood-Group System
/
Blood Grouping and Crossmatching
/
Base Sequence
/
Genetic Testing
/
Polymerase Chain Reaction
/
Exons
/
Alleles
/
Genotype
/
Mutation
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2015
Type:
Article