The effect of DNA methyltransferase 1 low expression on the global genome DNA methylation status of 16HBE cell / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 194-197, 2011.
Article
in Zh
| WPRIM
| ID: wpr-272668
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct DNA methyltransferase 1 (DNMT1) low expression 16HBE cell line and observe the variation of cell cycle and global genomic DNA methylation.</p><p><b>METHODS</b>The method of Lenti-virus induced RNA interference was applied to introduce four different shRNA fragment into 16HBE cells. Flow cytometry and 5-mC immunofluorescence methods were used to observe the cell cycle and global DNA methylation status of DNMT1 low expression 16HBE cells.</p><p><b>RESULTS</b>The DNMT1 protein relative expression level of 16HBE-shDNMT1-4 cell line was down regulated about 44% (P < 0.05) compared with the control. No obvious differences of cell cycle and global genome DNA methylation status were observed between the 16HBE and 16HBE-shDNMT1.</p><p><b>CONCLUSION</b>The DNMT1 gene low expression cell is successfully constructed, and there are no obvious changes happened on the cell cycle and global genomic DNA methylation.</p>
Full text:
1
Index:
WPRIM
Main subject:
Down-Regulation
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Cell Cycle
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Cell Line
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DNA Methylation
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RNA, Small Interfering
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RNA Interference
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DNA (Cytosine-5-)-Methyltransferases
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Epithelial Cells
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DNA (Cytosine-5-)-Methyltransferase 1
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Genetics
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2011
Type:
Article