Study on GC-MS fingerprint analysis in rhizome of volatile oil of Acorus tatarinowii / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the method of fingerprint analysis on volatile oil in rhizome of Acorus tatarinowii by GC-MS, and to study the main characteristic components.</p><p><b>METHOD</b>The main components of 10 samples were determined by GC-MS.</p><p><b>RESULT</b>The injector temperature was 250 degrees C. The interface temperature was 230 degrees C. The column flow was 1.3 mL x min(-1). The column pressure was 80 kPa. The detector volt was 1.4 kV. The temperature rate was 3 degrees C x min(-1). And the main characteristic components were composed of the methyleugenol (2.13%), cis-methylisoeugenol (4.48%), trans-methylisoeugenol (0.82%), gamma-asarone (4.51%), beta-asarone (66.15%), alpha-asarone (6.35%). And the RSD of precision and reproducibility and stability was almost in the range of 5%.</p><p><b>CONCLUSION</b>The method is reliable, accurate and can be used for fingerprint analysis of volatile oil of Acorus tatarinowii.</p>