Studies on callus growth and phillyrin accumulation of Forsythia suspensa / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of physical and chemical factors on callus growth and phillyrin contents of F. suspensa.</p><p><b>METHOD</b>The cell growth index and phyllirin yield in different culture condition such as different plant hormones mixed, mediums, light and dark were compared. HPLC was used to examine phillyrin contents.</p><p><b>RESULT AND CONCLUSION</b>Growth cycle of cells is twenty-eight days. During the course of callus growth, the processes of phillyrin biosynthesis were parallel with the cell growth. The optimum medium is MS. The optimum hormones concentrations are 1 mg.L-1 2,4-D, 0.5 mg.L-1 6-BA and 0.5 mg.L-1KT. The cell culture in light is more suitable than that in dark.</p>