Effect of HBP-A on meniscal injury and pathological hypertrophy and calcification of the meniscus / 南方医科大学学报
Journal of Southern Medical University
; (12): 431-437, 2016.
Article
in Zh
| WPRIM
| ID: wpr-273746
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of HBP-A on meniscal injuries and the expressions of genes associated with pathological hypertrophy and calcification of the meniscusinduced by abnormal loading.</p><p><b>METHODS</b>Bovine meniscus explants were subjected to 25% strain at 0.3 Hz for 3 h and treated with 0.6 mg/mL of HBP-A. The cell viability in the meniscus explants after 72 hin culture was determined using live/dead staining and the expression levels of genes associated with pathological hypertrophy and calcification of the meniscus (ANKH, ENPP1, ALP, MMP13, and IL-1) were measured using real-time PCR and Western blotting. The conditioned medium was collected for testing sulfated glycosaminoglycan (GAG) release.</p><p><b>RESULTS</b>The number of dead cells, loss of proteoglycan content, and the expressions of ANKH, ENPP1, ALP and MMP13, and IL-1 at both the mRNA and protein levels were all significantly lower in the meniscus explants treated with 0.6 mg/mL HBP-A than in the explants with only 25% abnormal pressure stimulation (n=3, P<0.05).</p><p><b>CONCLUSION</b>HBP-A can effectively alleviate meniscal injuries induced by abnormal loading and suppress the expressions of genes related with pathological hypertrophy and calcification of the meniscus, and can serve as a potential drug for treatment of knee osteoarthritis.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Menisci, Tibial
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Calcinosis
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Osteoarthritis, Knee
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Drug Therapy
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Real-Time Polymerase Chain Reaction
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Tibial Meniscus Injuries
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Glucans
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Hypertrophy
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2016
Type:
Article