Study of germline mutation of hMSH2, hMSH6 and hMLH1 and methylation of hMLH1 in microsatellite instability colorectal cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 358-361, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-273832
ABSTRACT
<p><b>OBJECTIVE</b>To study the characteristics of germline mutations of hMLH1, hMSH2 and hMSH6 and promoter methylation status of MLH1 in patients with MSI colorectal cancer.</p><p><b>METHODS</b>Sequence analysis of germline mutation and promoter methylation of MLH1 in 34 prospective collected patients with MSI colorectal cancer were performed.</p><p><b>RESULTS</b>Nineteen out of 34 patients with MSI colorectal cancer were detected with hypermethylation of MLH1,which accounted for 55.9%. 73.7% MSI-H colorectal cancer cases and 33.3% MSI-L colorectal cancer cases were detected with hypermethylation of MLH1 and the difference was significant. Eight germline mutations were found, including 3 MSH6 mutations and 5 MSH2 mutations.</p><p><b>CONCLUSION</b>There are some different characteristics of the germline mutations of hMLH1, hMSH2 and hMSH6 and promoter methylation of MLH1 in Chinese MSI colorectal patients.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Neoplasm
/
Nuclear Proteins
/
Colorectal Neoplasms
/
Sequence Analysis, DNA
/
Germ-Line Mutation
/
DNA Methylation
/
Base Pair Mismatch
/
Adaptor Proteins, Signal Transducing
/
DNA-Binding Proteins
/
MutS Homolog 2 Protein
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2008
Type:
Article
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