Determination of o-methylflavinantine in a Tibetan medicine Meconopsis quintuplinervia by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC method for the determination of a Tibetan medicine Meconopsis quintuplinervia.</p><p><b>METHOD</b>A Hypersil-Keystone-C18 column (4.6 mm x 250 mm, 5 microm) was used with the isocratic elution of acetonitrile and 0.012% glacial acetic acid. The flow rate was 1.0 mL x min(-1), and the detection wavelength was set at 237 nm.</p><p><b>RESULT</b>The linear range of 0-methylflavinantine was 0.2-2.4 microg (r = 0.999 7). The average recovery was 96.26%.</p><p><b>CONCLUSION</b>The developed method was reliable, and can be used for the quality control of M. quintuplinervia Regel.</p>