Expression and biological roles of heat shock protein 25 in rat dental follicle cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 492-496, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-274544
ABSTRACT
<p><b>OBJECTIVE</b>To examine the expression of heat shock protein 25 (HSP-25) in dental rat follicles in vivo and in vitro in order to investigate the possible effect of HSP-25 on cell proliferation and alkaline phosphatase (ALP) activity.</p><p><b>METHODS</b>The expression of HSP-25 in mandibles of postnatal rats from day 1, 3, 5, 7, 9, 11 was examined by immunohistochemistry in vitro, the expression of HSP-25 in the dental follicle cells was detected by the indirect immunofluorescence method. Methyl thiazolyl tetrazolium (MTT) assay, flowcytometry and ALP assay were used to detect the effect of HSP-25 on rat dental follicles.</p><p><b>RESULTS</b>HSP-25 expression was absent or weak in rat dental follicle cells at early postnatal stage and present from day 5 till day 11. HSP-25 was detected in the cytoplasm of cultured dental follicle cells. MTT results showed no effect could be detected on dental follicle cell proliferation after stimulation of different concentrations of HSP-25 on day 1, 2, 3. Flowcytometry results also exhibited no difference in cell cycles after stimulation of HSP-25 at 0 microg/L and 100 microg/L. HSP-25 at a concentration of 50 microg/L and 100 microg/L could up-regulate the ALP activity on day 9.</p><p><b>CONCLUSIONS</b>Expression of HSP-25 increases chronologically in the rat dental follicle cells. HSP-25 locates in the cytoplasm of cultured rat dental follicle cells. HSP-25 has no effect on the proliferation of dental follicle cells, however it can up-regulate the ALP activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Tetrazolium Salts
/
Thiazoles
/
Immunohistochemistry
/
Up-Regulation
/
Cells, Cultured
/
Fluorescent Antibody Technique, Indirect
/
Dental Sac
/
Cell Proliferation
/
Alkaline Phosphatase
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2009
Type:
Article
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