Development of the high resolution melting method for genotyping CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 477-481, 2012.
Article
in Zh
| WPRIM
| ID: wpr-275021
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the high-resolution melting curve (HRM) approach for genotyping CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) and explore its value on estimation of the Warfarin initial dose in comparison with various traditional genotyping methods.</p><p><b>METHODS</b>CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) genotyping was detected in 100 patients receiving Warfarin therapy by the newly developed HRM method and traditional genotyping methods including PCR-restriction fragment length polymorphism (PCR-RFLP), TaqMan probe and DNA sequencing.</p><p><b>RESULTS</b>The results of the genotypes obtained from above mentioned methods to detect CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) were similar and consistent. The HRM method is simpler, more economical, and faster compared to the traditional methods. The frequencies of the VKORC1-1639 AA, AG, GG genotypes from these 100 clinical samples were 73 (73%), 23 (23%), 4 (4%), respectively; Frequencies of the CYP2C9 1075 AA, AC, CC genotypes were 94(94%), 6 cases (6%), 0, respectively.</p><p><b>CONCLUSIONS</b>HRM approach can effectively detect CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) polymorphisms and this method is simpler, more economical, and faster compared to the traditional methods for detecting CYP2C9*3 (1075A/C, rs1057910) and VKORC1 (-1639A/G, rs9923231) polymorphisms.</p>
Full text:
1
Index:
WPRIM
Main subject:
Warfarin
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Polymorphism, Restriction Fragment Length
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Aryl Hydrocarbon Hydroxylases
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Therapeutic Uses
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Dose-Response Relationship, Drug
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Genotyping Techniques
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Vitamin K Epoxide Reductases
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Cytochrome P-450 CYP2C9
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Freezing
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Genetics
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Cardiology
Year:
2012
Type:
Article