Evaluation of the hydroxynitrile lyase activity in cell cultures of capulin (Prunus serotina) / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1199-1201, 2008.
Article
in English
| WPRIM
| ID: wpr-275403
ABSTRACT
Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The three week old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acetonitriles
/
Benzaldehydes
/
Cells, Cultured
/
Hydrogen Cyanide
/
Prunus
/
Cell Biology
/
Aldehyde-Lyases
/
Biocatalysis
/
Metabolism
/
Nitriles
Language:
English
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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