Construction of a novel gene therapy lentiviral vector for drug resistant selection and detection in vivo / 生物工程学报
Chinese Journal of Biotechnology
; (12): 256-261, 2008.
Article
in Zh
| WPRIM
| ID: wpr-276130
Responsible library:
WPRO
ABSTRACT
Lentiviral vectors were powerful gene delivery tools for gene therapy. We developed a new lentiviral vector pBobi-MIL that constitutively expressed O6-methylguanine-DNAmethyltransferase (MGMT) and Luciferase, linked by the internal ribosomal entry site (IRES), to realize drug tolerance and real time monitoring in vivo. All results from RT-PCR, drug treating clones forming, immunofluorometric assay and chemiluminescence detection showed that cells infected by recombinant lentivirus L-MIL simultaneously expressed these two genes. This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
Full text:
1
Index:
WPRIM
Main subject:
Drug Resistance
/
DNA Modification Methylases
/
Genetic Therapy
/
Lentivirus
/
Tumor Suppressor Proteins
/
DNA Repair Enzymes
/
Genetic Vectors
/
Genetics
/
Luciferases
/
Metabolism
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article