Puerarin inhibits DNA damage of HaCaT cells induced by UVB via ceramide pathway / 药学学报
Acta Pharmaceutica Sinica
; (12): 609-613, 2012.
Article
in Zh
| WPRIM
| ID: wpr-276272
Responsible library:
WPRO
ABSTRACT
This study is to investigate the effect and mechanism of puerarin on DNA damage of HaCaT cells induced by UVB. Puerarin pre-treated cells were irradiated with UVB at 30 mJ x cm(-2). Twenty four hours after irradiation, DNA damage was detected by comet assay, ceramide was measured by thin layer chromatography and gas chromatography, intracellular free calcium ion was analyzed by flow cytometry, the phosphorylation level of p38 protein was examined by Western blotting method. Levels of DNA damage, ceramide, free calcium ion and p-p38 protein were elevated in UVB model cells. Contrary to the model group, all indicators above were reduced in all groups pre-treated by puerarin. Puerarin restrains the ceramide accumulation to block downstream p38 MAPK pathway and calcium ion rising, therefore reduces DNA damage in HaCaT cells induced by UVB.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Phosphorylation
/
Radiation Effects
/
Ultraviolet Rays
/
DNA Damage
/
Signal Transduction
/
Down-Regulation
/
Keratinocytes
/
Cell Line
/
Ceramides
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2012
Type:
Article