Study on the effects of combined IL-12 and GM-CSF gene therapy for murine liver cancer / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 413-416, 2002.
Article
in Zh
| WPRIM
| ID: wpr-276548
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-tumor effects of combined IL-12 and granalocyte-macrophage-colong scimulating factor (GM-CSF) gene therapy on murine hepatocellular carcinoma.</p><p><b>METHODS</b>Twenty-four mice received subcutaneous inoculation of 1 x 10(6) BNL hepatoma cells were randomly divided into the following four groups with different cytokine encoding plasmids (6 mice for each group) (1)pXX-GM-CSF 12.5 microg and pXX-IL-12 12.5 microg; (2)pXX-IL-12 25 microg; (3)pXX-GM-CSF 25 microg; (4)pXX-Neo 25 microg. The plasmids were given through tail vein using a versatile hydrodynamics-based DNA delivery method on day 3 and day 6 after tumor challenge. The growth of tumor and cellular immune response were observed intensively. The changes in serum concentration of IL-12, GM-CSF, and IFN-gamma after plasmids injection were also observed.</p><p><b>RESULTS</b>Co-delivery of IL-12 and GM-CSF could mount stronger anti-tumor effects, longer term enhanced IL-12 expression and lower level of IFN-gamma than did IL-12 alone.</p><p><b>CONCLUSIONS</b>Combined IL-12 and GM-CSF can render a strong anti-tumor effect as well as a potential to lower the side effects.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
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Plasmids
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Therapeutics
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Time Factors
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Blood
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Tumor Cells, Cultured
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Genetic Therapy
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CD4-Positive T-Lymphocytes
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Cell Division
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Granulocyte-Macrophage Colony-Stimulating Factor
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article