A new method for isolating CD34(+) cells based on complex of magnetic nanoparticles and antibody / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 537-541, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-276877
ABSTRACT
The purpose of this study was to synthesize the complex of magnetic nanoparticles and antibody, and to apply them to isolate the CD34(+) cells from umbilical cord blood, then to evaluate its separation efficiency. The complex of magnetic nanoparticles and antibody was used to separate CD34(+) cells from umbilical cord blood in the outer magnetic field because of its superparamagnetism, specific identification and function of combination with CD34(+) cells. Scanning electron microscopy was used to observe the morphology of the separated CD34(+) cells. Flow Cytometer was applied to evaluate the sorting efficiency of magnetic nanoparticles, liquid culture and colony culture were taken to assay proliferation and differentiation capacity of the separated CD34(+) cells. The results showed that the CD34(+) cells from umbilical cord blood were isolated fast and effectively by the complex of magnetic nanoparticles and monoclonal antibody. Moreover, the isolated CD34(+) cells still maintained its normal morphology, highly proliferative and differentiative capacity. It is concluded that the complex of magnetic nanoparticles and monoclonal antibody has been successfully synthesized and developed as a technique which efficiently and quickly isolates CD34(+) cells from umbilical cord blood.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Hematopoietic Stem Cells
/
Immunomagnetic Separation
/
Antigens, CD34
/
Cell Biology
/
Allergy and Immunology
/
Nanoparticles
/
Fetal Blood
/
Flow Cytometry
/
Magnetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
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