Lidamycin induces apoptosis of human gastric carcinoma BGC823 cells and inhibits xenograft growth in nude mice / 药学学报
Acta Pharmaceutica Sinica
;
(12): 601-604, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-277789
ABSTRACT
To investigate the effect of lidamycin (LDM) on human gastric carcinoma BGC823 cells and xenograft growth in nude mice, MTT (methyl thiazolyl tetrazolium) assay was used to determine the inhibition of BGC823 cell proliferation by LDM. Induction of apoptosis was studied by flow cytometry and TUNEL assay. The expression of VEGF was detected by Western blotting analysis. Athymic nude mice were used to determine in vivo antitumor activity. Proliferation inhibition and apoptosis induction were studied in lidamycin-treated cells. The expression of VEGF in BGC823 cells decreased in a dose-dependent manner. LDM at 0.02 mg x kg(-1) and 0.04 mg x kg(-1) suppressed the growth of BGC823 xenografts in nude mice by 57% and 72%, respectively. LDM potently induces apoptosis in human gastric carcinoma BGC823 cells and inhibits xenograft growth.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Stomach Neoplasms
/
Random Allocation
/
Apoptosis
/
Xenograft Model Antitumor Assays
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Cell Proliferation
/
Dose-Response Relationship, Drug
Type of study:
Controlled clinical trial
/
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Type:
Article
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