Synthesis and mass spectrometric analysis of aristolochic acid-deoxyguanosine adducts / 药学学报
Acta Pharmaceutica Sinica
; (12): 295-298, 2008.
Article
in Zh
| WPRIM
| ID: wpr-277859
Responsible library:
WPRO
ABSTRACT
To synthesize aristolochic acid (AA)-2'-deoxyguanosine 5'-monophosphate (dGp) adducts in vitro and develop a novel method for the characterization of the adducts using multiple mass spectrometric techniques. AA was incubated with dGp in vitro using either enzymatic activation (by xanthine oxidase) or chemical activation (by zinc) to synthesize AA-dGp adducts, and the reaction conditions were optimized. Crude extracts were analyzed by techniques of liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-MS/MS) and high accuracy mass data and isotope pattern of super high resolution Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICRMS). The quasi-molecular ion peaks of the AA-dGp adducts were obtained in the negative ion mode. Analysis by electrospray ionization/tandem mass spectrometry (ESI-MS/MS) provided useful structural information about AA-dGp adducts. AA can bind covalently to the exocyclic amino group of deoxyguanosine to form AA-dGp adducts. MS analysis is a powerful tool to detect and identify AA-dGp adducts simply, rapidly and accurately.
Full text:
1
Index:
WPRIM
Main subject:
DNA
/
Chemistry
/
Chromatography, High Pressure Liquid
/
DNA Adducts
/
Aristolochic Acids
/
Deoxyguanosine
/
Tandem Mass Spectrometry
/
Metabolism
/
Methods
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Type:
Article