Construction of recombinant human CD59 using ICAM-2 promoter for endothelial-specific expression in xenotransplantation / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 588-591, 2002.
Article
in Zh
| WPRIM
| ID: wpr-278132
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant human CD59 gene containing intercellular adhesion molecule-2 promoter for high level endothelial-specific expression in xenotransplantation.</p><p><b>METHODS</b>ICAM-2 promotor fragment and CD59-intron 1 fragment were produced by PCR from the human blood genome, and then clone these fragments into a pcDNA3-CD59 eukaryotic expression vector which was followed by digestion with the specific restricted endonuclease (for example: EcoRI, Hind III). The ICAM-2 promoter and CD59-intron 1 fragments were identified by PCR, and sequencing. The recombinant was then transfected into pig aorta endothelial cells with Lipofection, and the expression was measured by flow cytometer.</p><p><b>RESULTS</b>Products of the sequences measured were in accord with the frames of the gene bank. The expression of the protein of this recombinant was positive.</p><p><b>CONCLUSION</b>The CD59 recombinant gene is constructed successfully, providing a basis for transgenic research.</p>
Full text:
1
Index:
WPRIM
Main subject:
Swine
/
Transplantation, Heterologous
/
Recombinant Proteins
/
Endothelium, Vascular
/
Transfection
/
Antigens, CD
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Cell Adhesion Molecules
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Gene Expression Regulation
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Promoter Regions, Genetic
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Cloning, Molecular
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2002
Type:
Article