Inclusion bodies of human cytomegalovirus are composed of the DNA and immediately early and early antigens of the virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 262-265, 2003.
Article
in Zh
| WPRIM
| ID: wpr-279582
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To study the composition and significance of the inclusion bodies of human cytomegalovirus (HCMV).</p><p><b>METHODS</b>Microdissection of inclusion bodies, PCR and Southern blot were adopted to detect DNA, and immunohistochemistry method and catalyzed signal amplification (CSA) were used to detect the different antigens of HCMV.</p><p><b>RESULTS</b>The inclusion bodies of HCMV were separated from the tissue section of human salivary gland. The fragments amplified by PCR from these dissected inclusion bodies were confirmed to be the DNA of HCMV. With the immunohistochemical method CSA, the immediately early and early antigens of HCMV were detected with monoclonal antibodies DDG9/CCH2, while matrix protein AAC10 was negative in the inclusion bodies.</p><p><b>CONCLUSION</b>The ingredient of inclusion bodies of HCMV included the DNA and the antigens expressed in specific stage of the virus.</p>
Full text:
1
Index:
WPRIM
Main subject:
Salivary Glands
/
Virology
/
DNA, Viral
/
Immunohistochemistry
/
Inclusion Bodies
/
Chemistry
/
Cytomegalovirus Infections
/
Cytomegalovirus
/
Microdissection
/
Diagnosis
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2003
Type:
Article