Effect of caspase 9 related signaling molecules on the apoptosis of human vascular endothelial cell induced by homocysteine / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 383-386, 2005.
Article
in English
| WPRIM
| ID: wpr-280045
ABSTRACT
<p><b>OBJECTIVE</b>To understand the role of mitochondria associated signaling pathway in the apoptosis of human vascular endothelial cell induced by homocysteine (Hcy).</p><p><b>METHODS</b>The mRNA and protein expression levels of the up-stream signaling molecules of caspase 3, Bcl 2, caspase 9, and cytosolic cytochrome-c, were investigated. The in vitro cultured human umbilical vein endothelial cells with homocysteine at different concentrations were incubated for 24 h. The expressions of Bcl 2 and caspase 9 at mRNA and protein levels were analyzed by reverse transcription-polymerase chain reaction(RT-PCR) and Western blot. Cytochrome-c in cytoplasm was also detected by Western blot.</p><p><b>RESULTS</b>The expression levels of three signaling molecules were all down-regulated by homocysteine at both mRNA and protein levels in a dose-dependent manner.</p><p><b>CONCLUSION</b>Homocysteine could affect the formation of apoptosome through repressing the expression of Bcl 2 gene and release of cytochrome-c from mitochondria. Decreasing of apoptosome could disturb the activation of caspase 9. The results also indicate that the mitochondria pathway is not the major signaling pathway involved in Hcy-induced apoptosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Signal Transduction
/
Cells, Cultured
/
Blotting, Western
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
/
Endothelial Cells
/
Cytochromes c
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Medical Genetics
Year:
2005
Type:
Article
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