Detection of fusion genes associated with specific translocations in acute leukemia patients with normal karyotypes by using multiplex RT-PCR / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 228-231, 2006.
Article
in Zh
| WPRIM
| ID: wpr-280695
Responsible library:
WPRO
ABSTRACT
This study was aimed to explore the usefulness of multiplex reverse transcription-polymerase chain reaction (multiplex RT-PCR) in detection of fusion genes associated with specific translocations in acute leukemia (AL) patients with normal karyotypes. 37 AL patients with normal karyotypes were analyzed by multiplex RT-PCR. The results showed that 4 types of fusion genes such as PML/RARA, AML1/ETO, CBFbeta/MYH11, BCR/ABL were detected in 8 (21.6%) patients by multiplex RT-PCR. In conclusion, multiplex RT-PCR is useful in detection of fusion genes associated with specific translocations in acute leukemia (AL) with normal karyotypes and it would refine the karyotype analysis. When the normal karyotypes were detected in acute leukemia patients by conventional cytogenetic method, the multiplex RT-PCR should be performed for them.
Full text:
1
Index:
WPRIM
Main subject:
Prognosis
/
Translocation, Genetic
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Leukemia, Myeloid, Acute
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Leukemia
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Oncogene Proteins, Fusion
/
Acute Disease
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cytogenetic Analysis
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
Genetics
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Adolescent
/
Adult
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Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article