Expression of HOXB4 in cord blood progenitor cells expanded in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 89-93, 2006.
Article
in Zh
| WPRIM
| ID: wpr-280727
Responsible library:
WPRO
ABSTRACT
HOXB4, a member of homeobox gene family, is closely related to the self-renewing and proliferative ability of primitive hematopoietic stem/progenitor cells (PHSC/PHPC). This study was aimed to investigate the self-renewing level of cord blood progenitor cells (CBPC) expanded in vitro. The HOXB4 expression at mRNA level was assayed by using real time RT-PCR. The results indicated that as culture prolonged, the total cells, CD34(+) cells greatly increased, however the HOXB4 expression gradually declined, even down to undetectable level similar to that of mature lymphocytes. Meanwhile, it was shown that CD34(+) cells co-cultured with bone marrow mesenchymal stem cells (BM-MSC) could abate the decline of HOXB4 expression. It is concluded that the self-renewing potential of CD34(+) cells gradually decreased during expansion in vitro, co-culture with BM-MSC was helpful to CD34(+) cell expansion and slowed the loss trend of its self-renewal.
Full text:
1
Index:
WPRIM
Main subject:
Transcription Factors
/
Bone Marrow Cells
/
Cells, Cultured
/
Homeodomain Proteins
/
Coculture Techniques
/
Antigens, CD34
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Cell Biology
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Cell Proliferation
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Fetal Blood
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Mesenchymal Stem Cells
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article