Inhibition of proliferation and induction of apoptosis and differentiation of leukemic cell line HL-60 by sodium valproate / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 21-24, 2006.
Article
in Zh
| WPRIM
| ID: wpr-280742
Responsible library:
WPRO
ABSTRACT
To investigate the influence of sodium valproate (VPA) on proliferation, apoptosis and differentiation of HL-60 cell line, effect of VPA in various concentrations on proliferation of HL-60 cells was detected by MMT; Wright-Giemsa staining was performed to observe the morphologic changes of HL-60 cells; NBT experiment was used to test the differentiation of HL-60 cells; flow cytometry was used to observe cell cycles and analyze the apoptosis. The results indicated that the changes of the growth curve showed inhibition of proliferation of HL-60 cells. After a 24-48 hours culture with 2 mmol/L VPA, the cells exhibited nuclear shrinkage, pyknosis fragmentation and appearance of apoptosis bodies. The percentage of the annexin V(+)/PI(-) cells which were apoptotic increased from 2.9% to 17.1%; hypodiploid peak was observed; the percentage of HL-60 cells in G(1) phase increased from 51.1% to 84.6% and the cells in S phase decreased from 37.9% to 14.4%. After a week culture with 0.25 mmol/L VPA, the cells exhibited characteristics of differentiation. The percentage of NBT positive cells was (47 +/- 2)%. It is concluded that VPA can inhibit the proliferation of HL-60 cells while inducing differentiation and apoptosis of these cells. The mechanism needs to be further studied.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Cell Cycle
/
Cell Differentiation
/
Valproic Acid
/
Apoptosis
/
HL-60 Cells
/
Cell Proliferation
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2006
Type:
Article