Prophylactic protective effects of paeoniflorin on corticosterone-induced primary cultures of rat cortical neurons damages / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the prophylactic neuroprotective effect of paeoniflorin (PF) on corticosterone-induced primary cultures of rat cortical neurons damages.</p><p><b>METHOD</b>Primary cultures of neurons from the of 16-days-old embryo rats were used to study. Neurons in the experimental group were treated with cort (200 micromol x L(-1)) 30 min after the pretreatment of PF (0.5, 2, 10 micromol x L(-1)). We used MTT assay to detect the action of paeoniflorin on neuronal activity. DAPI staining to study of the influence of PF on neuronal apoptosis morphology, and Annexin V-FITC/PI apoptosis detection kit to inspect the impact of paeoniflorin on the early neuronal apoptosis.</p><p><b>RESULT</b>Compared with model group, every dose of PF (0.5, 2, 10 micromol x L(-1)) can raise the neuronal activity notably (P<0.05), PF of 2 micromol x L(-1) and 10 micromol x L(-1) can reduce the rate of neuronal apoptosis.</p><p><b>CONCLUSION</b>Our data indicated that PF achieved prophylactic neuroprotective effect through against corticosterone-induced neurons damages.</p>