Simultaneous determination of five active constitutents in Xiaochaihu Tang by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 708-710, 2010.
Article
in Zh
| WPRIM
| ID: wpr-281734
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC-PDA method for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang.</p><p><b>METHOD</b>A Symmetry Shield RP18 (4.6 mm x 250 mm, 5.0 microm) was used with a mobile phase of acetonitrile-0.01% H3PO4 in gradient elution. The detection wavelength was 251 nm,the flow rate was 0.45 mL x min(-1) and the column temperature was maintained at 30 degrees C.</p><p><b>RESULT</b>The accuracy, precision, sensitivity, specificity and linearity of this method met the requirements. The contents of the five effective fractions were determined simultaneously.</p><p><b>CONCLUSION</b>The method is rapid,simple and accurate and it can be suitable for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.</p>
Full text:
1
Index:
WPRIM
Main subject:
Spectrophotometry, Ultraviolet
/
Flavonoids
/
Drugs, Chinese Herbal
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Technology, Pharmaceutical
/
Glycyrrhizic Acid
/
Rehmannia
/
Flavanones
/
Glucosides
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2010
Type:
Article