Effect of nordy on FPR function of malignant human glioma cell line U87 / 药学学报
Acta Pharmaceutica Sinica
; (12): 257-262, 2007.
Article
in Zh
| WPRIM
| ID: wpr-281911
Responsible library:
WPRO
ABSTRACT
Nordy is a synthesized chrial compound. To investigate the effects of nordy (25 - 100 micromol x L(-1)) on the function of formylpeptide receptor (FPR) of malignant human glioma cells, human glioblastoma cell line U87 was used to detect its proliferation, migration, calcium mobilization, vascular endothelial growth factor (VEGF) mRNA and protein levels after activation of FPR by its agonist N-formyl-methionyl-leucyl-phenylalanine (fMLF). Cell proliferation, migration ability, VEGF mRNA, VEGF protein and calcium mobilization were evaluated by cell counting, chemotaxis assay, RT-PCR, ELISA and spectrometry. Nordy (50 - 100 micromol x L(-1)) potently inhibited the proliferation, migration and calcium mobilization of U87 cells induced by fMLF (P < 0.05). Moreover, 100 micromol x L(-1) nordy showed a significantly impaired VEGF mRNA expression and protein secretion induced by fMLF (P < 0.05). Nordy could inhibit FPR functioning in glioma cell proliferation, migration and angiogenesis, which might be a possible mechanism of its anti-cancer effects.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Spectrophotometry
/
Masoprocol
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Cell Movement
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Calcium
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Glioblastoma
Limits:
Humans
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2007
Type:
Article