Detection of EWS-FLI1 fusion transcript in Ewing's sarcoma/peripheral primitive neuroectodermal tumors by one-step RT-PCR using paraffin-embedded tissues / 中华病理学杂志
Chinese Journal of Pathology
; (12): 328-331, 2004.
Article
in Zh
| WPRIM
| ID: wpr-283514
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of EWS-FLI1/ERG fusion transcript resulting from t(11;12)(q24;12) in paraffin-embedded tissues and its diagnostic implication for Ewing's sarcoma/peripheral primitive neuroectodermal tumors (ES/pPNET).</p><p><b>METHODS</b>One-step reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect a characteristic EWS-FLI1/ERG fusion transcript in 25 cases of ES/pPNET and 15 cases of other small round cell tumors (including 8 cases of rhabdomyosarcoma, 4 cases of synovial sarcoma, 2 cases of neuroblastoma and 1 case of lymphoma) using formalin-fixed and paraffin-embedded tissues.</p><p><b>RESULTS</b>EWS-FLI1/ERG fusion transcript was detected in 20 of the 25 ES/pPNET cases (80%). The 15 non-ES/pPNET control cases were negative for EWS-FLI1/ERG fusion transcript.</p><p><b>CONCLUSIONS</b>Detection of EWS-FLI1/ERG fusion transcript is a reliable index for molecular diagnosis of ES/pPNET. One-step RT-PCR is a practical method for such analysis in routine paraffin-embedded tumor tissues.</p>
Full text:
1
Index:
WPRIM
Main subject:
Rhabdomyosarcoma
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Sarcoma, Ewing
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Transcription Factors
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Oncogene Proteins, Fusion
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Paraffin Embedding
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Neuroectodermal Tumors, Primitive, Peripheral
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Sarcoma, Synovial
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Reverse Transcriptase Polymerase Chain Reaction
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RNA-Binding Protein EWS
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Diagnosis
Type of study:
Diagnostic_studies
Limits:
Adolescent
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Adult
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2004
Type:
Article