Rapid determination of contents of hirudin's hydrolysates in processed leech by dot blotting / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2193-2195, 2008.
Article
in Zh
| WPRIM
| ID: wpr-283767
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the contents of hirudin's hydrolysates in processed leeches, set up a new evaluating method of dot blotting to evaluate the qualities of those processed Chinese medicines as leeches.</p><p><b>METHOD</b>Contents of hirudin's hydrolysates in processed leeches were determined by dot blotting with rat antibody of anti-hirudin as the first antibody. Blotting signal was analyzed by software of Quantity One.</p><p><b>RESULT</b>Contents of hirudin's hydrolysates in four batches of processed leeches were 296.51, 165.47, 95.58, and 298.05 microg g(-1), respectively.</p><p><b>CONCLUSION</b>Difference among four batches of processed leeches was significant in the content of hirudin's hydrolysates. Dot blotting, as a convenient and accurate method can be broadly used for evaluating processed products of Chinese crude drugs similar to leeches.</p>
Full text:
1
Index:
WPRIM
Main subject:
Drugs, Chinese Herbal
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Immunoblotting
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Chemistry
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Reproducibility of Results
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Hirudins
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Allergy and Immunology
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Leeches
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Metabolism
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Methods
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article