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The role of interleukin-18 and interleukin-18 receptor in predominant Th1 immune response of patients with immune thrombocytopenia / 中华血液学杂志
Chinese Journal of Hematology ; (12): 658-661, 2009.
Article in Zh | WPRIM | ID: wpr-283922
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the role of interleukin (IL)-18 and IL-18 receptor (IL-18R) in the predominant Th1 type cytokine response in patients with immune thrombocytopenia (ITP).</p><p><b>METHODS</b>Fifteen patients with active phase ITP, eighteen in remission and thirteen healthy controls were enrolled in this study. T-bet and GATA-3 mRNA levels in peripheral blood mononucleated cells (PBMNC) were measured by reverse transcriptase polymerase chain reaction (RT-PCR); the plasma IL-18 level by enzyme linked immunosorbent assay (ELISA), the expression of IL-18R on CD3(+) lymphocytes and total lymphocytes by flow cytometry(FCM).</p><p><b>RESULTS</b>The T-bet mRNA levels in patients with active phase ITP was 3.572 fold as much as that in the controls (P < 0.05), while the GATA-3 mRNA levels were 0.378 fold of that in controls (P < 0.05). The levels of plasma IL-18 and IL-18R on CD3(+) lymphocytes were significantly increased in active phase ITP than in remission phase and controls. There was no difference in ratio of T-bet/GATA-3 between remitted ITP and controls and so was for T-bet mRNA, GATA-3 mRNA, plasma IL-18 and IL-18R on CD3(+) lymphocytes.</p><p><b>CONCLUSION</b>ITP as a disease of Th1-dominant response there is an unbalance between T-bet and GATA-3 in its active phase; IL-18 and IL-18R being upregulated.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Case-Control Studies / Purpura, Thrombocytopenic, Idiopathic / Th1 Cells / Interleukin-18 / T-Box Domain Proteins / Allergy and Immunology / GATA3 Transcription Factor / Receptors, Interleukin-18 / Metabolism Type of study: Observational_studies Limits: Adolescent / Adult / Aged / Female / Humans / Male Language: Zh Journal: Chinese Journal of Hematology Year: 2009 Type: Article
Full text: 1 Index: WPRIM Main subject: Case-Control Studies / Purpura, Thrombocytopenic, Idiopathic / Th1 Cells / Interleukin-18 / T-Box Domain Proteins / Allergy and Immunology / GATA3 Transcription Factor / Receptors, Interleukin-18 / Metabolism Type of study: Observational_studies Limits: Adolescent / Adult / Aged / Female / Humans / Male Language: Zh Journal: Chinese Journal of Hematology Year: 2009 Type: Article