Anti-human 4-1BBL monoclonal antibody stimulates the nuclear translocation of NF-κB and the co-location of 4-1BBL/CD28 isoform in U937 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1121-1125, 2013.
Article
in Zh
| WPRIM
| ID: wpr-283969
Responsible library:
WPRO
ABSTRACT
This study was purposed to investigate the molecular mechanism of 4-1BBL reverse signals in the human acute monocytic leukemia cell line of U937. The U937 cell line was used as target cells, and stimulated by the mouse anti-human 4-1BBL monoclonal antibody 1F1. The nuclear translocation of NF-κB and the co-location of 4-1BBL and CD28i molecules in U937 cells were observed with confocal laser scanning microscopy. The protein and m-RNA expression levels of 4-1BBL and CD28i were detected by flow cytometry and RT-PCR respectively. The results showed that the significant nuclear translocation of NF-κB and co-localization of 4-1BBL and CD28i on membrane of U937 cells appeared after being stimulated by mAb1F1. It is concluded that the 4-1BBL reverse signals transduction mediating the growth of U937 cells relates with the nuclear translocation of NF-κB. CD28i may be involved in intracellular 4-1BBL reverse signaling pathways.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Signal Transduction
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NF-kappa B
/
CD28 Antigens
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Coculture Techniques
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U937 Cells
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Allergy and Immunology
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4-1BB Ligand
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Genetics
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Metabolism
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article