Establishment of a high-throughput respiratory virus detection technology without RNA purification and reverse transcription / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 24-28, 2013.
Article
in Zh
| WPRIM
| ID: wpr-284310
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring.</p><p><b>METHODS</b>We used high-throughput microsphere-based flexible multi-analyte profiling technology (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated.</p><p><b>RESULTS</b>There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies.</p><p><b>CONCLUSION</b>We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.</p>
Full text:
1
Index:
WPRIM
Main subject:
Influenza A virus
/
Influenza B virus
/
Respiratory Syncytial Viruses
/
Respiratory System
/
Virology
/
RNA, Viral
/
Sensitivity and Specificity
/
Respirovirus
/
Nucleic Acid Amplification Techniques
/
Metapneumovirus
Type of study:
Diagnostic_studies
Language:
Zh
Journal:
Acta Academiae Medicinae Sinicae
Year:
2013
Type:
Article