Study on the differentially expressed genes in bone tissue of ovariectomized rats after intervention with Chinese herbs / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the therapeutic effect and mechanism of jiangu granule (JGG) on postmenopausal osteoporosis.</p><p><b>METHODS</b>Differential display reverse transcription PCR (DDRT-PCR) was used to detect the differentially expressed genes in bone tissues associated with therapeutic effect of JGG.</p><p><b>RESULTS</b>Ten differentially expressed gene fragments were found, 9 of them, after cloning, sequencing and BLAST searching, were proved to be matched with highly homologous sequences in Genebank. Among them, two were known proteins hyaluronan-mediated motility receptor (RHAMM) and ATPase, Na+, K+ transporting beta 3 polypeptide (ATP1b3). It was confirmed by semi-quantitative RT-PCR that these two gene fragments could be down- and up-regulated by JGG respectively.</p><p><b>CONCLUSION</b>The therapeutic effect of JGG in treating postmenopausal osteoporosis might be related with its regulation on the two kinds of protein, RHAMM and ATP1b3.</p>