Association analysis of the parkin gene in patients with sporadic Parkinson's disease from a Han population of Sichuan province / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 38-41, 2007.
Article
in Zh
| WPRIM
| ID: wpr-285037
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether there are any associations between the -258T/G polymorphism of the promoter and the IVS3 -20T/C polymorphism in parkin gene and Parkinson's disease (PD) from a Han population in Sichuan province.</p><p><b>METHODS</b>Polymerase chain reaction (PCR), restriction fragment length polymorphism, denaturing high performance liquid chromatography(dHPLC) and sequence analysis were used to determine the genotype of each subject. The -258T/G polymorphism and IVS3 -20T/C polymorphism were analysed in 198 patients with sporadic PD and 187 healthy controls, matched for age and gender.</p><p><b>RESULTS</b>There were significant differences in allele frequency of the -258T/G polymorphism between PD patients and controls, with the G allele more common in cases than controls (52.5% vs 43.3%; chi square is 6.17, P< 0.025, OR is 1.45, 95% CI 1.04-1.86). There were also significant differences in G allele frequency between PD patients with onset age over 50 years old and controls(chi square is 9.048, P< 0.01, OR is 1.57, 95% CI1.08-2.06). The frequency of TG+GG genotype was significantly higher in PD patients than in controls (78.79% vs 69.51%; chi square is 3.854, P< 0.05, OR is 1.63, 95% CI0.88-2.38). In addition, there were significant differences in age of onset between PD patients with different genotypes (P< 0.05). The average age of onset in group of GG genotype was later about 5 years compared with the group of TT or TG genotype. The frequency of CC genotype in IVS3 -20T/C polymorphism was much higher than that of TC genotype. No TT genotype was found.</p><p><b>CONCLUSION</b>This study suggests that the parkin promoter -258T/G polymorphism might be a risk factor for late onset PD in Sichuan. CC genotype for IVS3 -20T/C polymorphism is common in Sichuan Han population. No TT genotype for IVS3 -20T/C polymorphism is found in Sichuan Han population.</p>
Full text:
1
Index:
WPRIM
Main subject:
Parkinson Disease
/
Polymorphism, Genetic
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Polymorphism, Restriction Fragment Length
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China
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Polymerase Chain Reaction
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Chromatography, High Pressure Liquid
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Promoter Regions, Genetic
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Genetic Predisposition to Disease
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Ubiquitin-Protein Ligases
/
Asian People
Type of study:
Risk_factors_studies
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
/
Male
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Type:
Article