Characteristics of IGF-II gene imprinting in twin placentas / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 497-501, 2006.
Article
in Zh
| WPRIM
| ID: wpr-285092
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare insulin-like growth factor II (IGF-II) gene imprinting in twin placentas with singleton ones and to determine whether imprinting was influenced by assisted reproductive technology, zygosity and fetal sex.</p><p><b>METHODS</b>One hundred and sixty cases of twin placentas and 42 cases of singleton ones were recruited. Allele-specific IGF-II expression was determined by reverse transcription-PCR combined with analysis of an Apa I-sensitive restriction fragment length polymorphism.</p><p><b>RESULTS</b>Although the incidence of IGF-II imprinting loss was higher in normal twin placentas than in singleton ones (20.6% vs 8.7%), there was no statistical significance. There were no significant differences between twins conceived by assisted reproductive technology and those conceived spontaneously (17.9% vs 24.4%), and between dizygotic and monozygotic twins (22.4% vs 16.7%). The incidence of IGF-II imprinting loss in placenta of female twins was statistically higher than that of male ones (26.4% vs 9.8%).</p><p><b>CONCLUSION</b>The risk of IGF-II gene imprinting loss is higher in female twins and has no relationship with assisted reproductive technology and zygosity.</p>
Full text:
1
Index:
WPRIM
Main subject:
Placenta
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Polymorphism, Genetic
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Twins
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Insulin-Like Growth Factor II
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Maternal Age
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Genomic Imprinting
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Reverse Transcriptase Polymerase Chain Reaction
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Genetics
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Metabolism
Limits:
Adult
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Female
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Humans
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Male
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Newborn
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Pregnancy
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2006
Type:
Article