Identification of proteins interacted with Bat3 using tandem affinity purification / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To identify the specific protein interactions involved in Bat3-mediated apoptosis.</p><p><b>METHODS</b>Tandem affinity purification (TAP) was utilized to investigate Bat3-protein interactions, during which full-length human Bat3 fused with Strep2 and FLAG tag as a bait was used to screen the specific protein-protein interactions. The isolated proteins were identified with mass spectrometry.</p><p><b>RESULTS</b>TAP studies showed that Ubl4A was identified as a Bat3-binding partner. Further investigation using co-immunoprecipitation confirmed that Bat3 was associated with Ubl4A.</p><p><b>CONCLUSION</b>TAP was successfully established and is suitable for isolating the binding partners of Bat3.</p>