Expression and characterization of rabies virus nucleoprotein in baculovirus / 病毒学报
Chinese Journal of Virology
; (6): 351-356, 2010.
Article
in Zh
| WPRIM
| ID: wpr-286113
Responsible library:
WPRO
ABSTRACT
To construct a recombinant expression plasmid Bacmid-N containing the N gene of Rabies Virus, the N gene of RV CVS-11 strain was cloned into the baculovirus shuttle vector (Bacmid). Recombinant Baculovirus AcMNPV-N (P1 Viral stock) was obtained by transfecting the Bacmid-N into the insect cell line of Sf9. The expressed nucleoprotein was identified and analysised by ELISA, FA, SDS-PAGE and Western blot assays. The results showed that the NP protein was expressed intracellularly and had a good antigenicity, which would be potentially used for further study on the diagnostic reagent of rabies virus detection.
Full text:
1
Index:
WPRIM
Main subject:
Rabies virus
/
DNA, Viral
/
Enzyme-Linked Immunosorbent Assay
/
Cell Line
/
Baculoviridae
/
Spodoptera
/
Fluorescent Antibody Technique, Indirect
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Virology
Year:
2010
Type:
Article