Expression of AIV subtype H5HA, H7HA and H9HA hemagglutinin gene in Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 231-236, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-286303
ABSTRACT
The expression of the hemagglutinins of Avian influenza virus H5 H7and H9 subtypes was studied in this article by Pichia pastoris, one of the eukaryotis expression systems. Three reconstructed expression plasmids and engineering strains, named pPIC9K-H5HA, pPIC9K-H7HA, pPIC9K-H9HA and GS115/pPIC9K-H5HA, GS115/pPIC9K-H7HA, GS115/pPIC9K-H9HA repectively, were obtained. The reconstructed yeast engineering strains were identified by MD and MM plate selecting and PCR. The induced interests proteins were examined by SDS-PAGE and Western-bloting,the results showed that the interest genes were expressed exactly. And this will be helpful in the future study of antigen detection and antibody detection kit, as well in the subunit vaccines developing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pichia
/
Hemagglutinin Glycoproteins, Influenza Virus
/
Influenza A Virus, H5N1 Subtype
/
Influenza A Virus, H7N7 Subtype
/
Influenza A Virus, H9N2 Subtype
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2006
Type:
Article
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