Effect of genistein on expression and current of voltage-gated calcium channels in mouse pancreatic beta-cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 206-210, 2005.
Article
in Zh
| WPRIM
| ID: wpr-287056
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To determine the effect of long-term inactivation of tyrosine kinases on voltage-gated calcium currents in pancreatic beta-cells and to evaluate the function of tyrosine kinases in pancreatic beta-cells.</p><p><b>METHODS</b>Primarily cultured mouse pancreatic islets and beta-cells were pretreated by 0.1 mmol/L genistein for 12 hours. Voltage-gated calcium currents and action potentials were recorded with patch clamp techniques in the configuration of perforated whole-cell recording. RT-PCR method was used to evaluate the changes in expression of voltage-gated calcium channels alpha1 subunit.</p><p><b>RESULTS</b>After treatment by genistein for 12 hours, the whole-cell voltage-gated calcium currents were significantly diminished (-13.83 +/- 1.515 pA/pF vs. -7.012 +/- 1.502 pA/pF, P < 0.01, n=6). The amplitudes of action potentials in genistein-treated beta-cells were also significantly attenuated (38.50 +/- 7.46 mV vs. 15.95 +/- 4.39 mV, P < 0.01, n=6). The expression of voltage-gated calcium channels alpha1 subunit in mouse islets was significantly decreased to 0.792 +/- 0.078 of that in control conditions (P < 0.01, n=5).</p><p><b>CONCLUSION</b>Genistein treatment decreases expression and current of voltage-gated calcium currents in mouse pancreatic beta-cells, which suggests that inhibition of tyrosine kinases activity plays an important role in the dysfunction of pancreatic beta-cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Physiology
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Action Potentials
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Cells, Cultured
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Patch-Clamp Techniques
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Genistein
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Potassium Channels, Calcium-Activated
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Insulin-Secreting Cells
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Metabolism
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Mice, Inbred C57BL
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2005
Type:
Article