Determination of four anthraquinones in Sanhuang tablets by QAMS / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a quality evaluation method, quantitative analysis of multi-component with a single-marker (QAMS) to determine the contents of four rhubarb anthraquinones in Sanhuang tablets.</p><p><b>METHOD</b>Emodin was used as the internal reference substance, the relative correlation factors (RCF) of rhein, chrysophanol and physcion to emodin were calculated and evaluated. The contents of these four anthraquinones were determined by the external standard method and QAMS, respectively. Rationality, feasibility and repeatability of the QAMS method was verified by comparing the results obtained from the two different methods.</p><p><b>RESULT</b>RCFs of rhein, chrysophanol and physcion to emodin against Sanhuang tablets were 1.13, 1.46 and 1.01, respectively, indicating good reproducibilities. The two methods did not show significant difference in results.</p><p><b>CONCLUSION</b>The QAMS method can be used a new quality assessment model for quantity of anthraquinones in Sanhuang tablets.</p>