Isolation of bone marrow-derived rat osteoclast-like cells with the digestion of trypsin / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 350-361, 2006.
Article
in Zh
| WPRIM
| ID: wpr-288930
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To obtain highly enriched osteoclasts in vitro.</p><p><b>METHODS</b>The bone marrow cells of 1-day, 12-day, 24-day and 36-day old Sprague-Dawley (SD) rats were separately cultured with the presence of 1,25-(OH)2D3 and dexamethasone, and the osteoclast-like cells were identified by Trap staining and scanning electron microscope observation and were purified by trypsin digestion. The count of Trap positive osteoclast-like cells was analyzed statistically.</p><p><b>RESULTS</b>The osteoclast-like cells of each group were Trap positive cells and could form the bone absorption lacunas in vitro. The amounts of osteoclast-like cells were different statistically between the groups (P < 0.05) except between the groups of 1-day old and 12-day old (P > 0.05). Highly enriched osteoclast-like cells were harvested by the digestion of 0.25% trypsin and 0.02% EDTA.</p><p><b>CONCLUSION</b>It's indicated that a large amount of highly enriched osteoclast-like cells could be obtained through the culture of bone marrow cells of 10-day and 12-day old SD rats with 1,25-(OH)2D3 and dexamethasone and the digestion of trypsin/EDTA.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteoclasts
/
Bone and Bones
/
Bone Marrow
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Calcitriol
/
Bone Marrow Cells
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Dexamethasone
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Trypsin
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Cells, Cultured
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Rats, Sprague-Dawley
Limits:
Animals
Language:
Zh
Journal:
West China Journal of Stomatology
Year:
2006
Type:
Article