VEGF antisense oligonucleotide inhibits the expression of vascular endothelial growth factor in human leukemic cell lines / 中华血液学杂志
Chinese Journal of Hematology
; (12): 22-25, 2004.
Article
in Zh
| WPRIM
| ID: wpr-291459
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of vascular endothelial growth factor (VEGF) antisense phosphorothioated oligodeoxynucleotide (AS-ODN) on the expression of VEGF in human leukemic cell lines (HL-60 and K562 cells).</p><p><b>METHODS</b>The levels of VEGF mRNA and protein in leukemic cells incubated with VEGF AS-ODN were measured by RT-PCR, immunohistochemistry assay and ELISA. MTT test was used to examine the influence of the culture supernatant (CS) of VEGF AS-ODN treated leukemic cells on the proliferation of human umbilical vein endothelial cells (ECV304).</p><p><b>RESULTS</b>After leukemic cells were treated with different concentrations (2.5 approximately 15.0 micro mol/L) of VEGF AS-ODN for 24 h, VEGF mRNA level in the cells decreased remarkably in a concentration dependent manner, no change was found in the VEGF missense ODN treated cells (MS-ODN). When the leukemic cells were treated with 5 micro mol/L VEGF AS-ODN for 24 h, VEGF protein level decreased greatly both in the cells and in the CS; and the proliferation stimulating effect of the treated CS on the ECV304 cells reduced. Meanwhile, there was no obvious change in VEGF protein and its effect in the VEGF MS-ODN treated group.</p><p><b>CONCLUSION</b>VEGF AS-ODN could inhibit VEGF expression in human leukemic cell lines in vitro.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
RNA, Messenger
/
Enzyme-Linked Immunosorbent Assay
/
Immunohistochemistry
/
Oligonucleotides, Antisense
/
HL-60 Cells
/
K562 Cells
/
Vascular Endothelial Growth Factor A
/
Genetics
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article