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Clonal expansion of TCR Vbeta subfamily T cells induced by bcr3-abl2 peptide / 中华血液学杂志
Chinese Journal of Hematology ; (12): 95-99, 2004.
Article in Chinese | WPRIM | ID: wpr-291467
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clonal expansion of T cell receptor (TCR) Vbeta subfamily T cells from cord blood induced by bcr3-abl2 peptide in vitro.</p><p><b>METHODS</b>T cells from 3 units of cord blood were amplified by anti-CD(3) monoclonal antibody (McAb) and IL-2 with or without synthetic b3a2 peptide. T cell specific cytotoxicity was analyzed by lactate dehydrogenase (LDH) assay, TCR Vbeta subfamilies by using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan technique.</p><p><b>RESULTS</b>bcr3-abl2 peptide specific cytotoxicity T cells were successfully induced from the 3 units of cord blood by synthetic b3a2 peptide. Compared with that in CD(3) McAb induced cells, distribution pattern of TCR Vbeta repertoire was different in T cells induced with b3a2 peptide. Oligoclonal and oligoclonal tendency TCR Vbeta subfamily T cells could be identified in cord blood T cells induced by b3a2 peptide in 1 or 2 weeks, whereas those induced by anti-CD(3) McAb and IL-2 were mostly polyclonal.</p><p><b>CONCLUSION</b>The cytotoxicity T cells with anti-CML specificity could be induced by b3a2 peptide. The specific anti-CML cytotoxicity may be derived from the clonal expansion TCR Vbeta subfamily T cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / T-Lymphocytes, Cytotoxic / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Fusion Proteins, bcr-abl / Interleukin-2 / CD3 Complex / Genes, T-Cell Receptor beta / Allergy and Immunology / Antibodies, Monoclonal Type of study: Prognostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Hematology Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / T-Lymphocytes, Cytotoxic / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Fusion Proteins, bcr-abl / Interleukin-2 / CD3 Complex / Genes, T-Cell Receptor beta / Allergy and Immunology / Antibodies, Monoclonal Type of study: Prognostic study Limits: Humans Language: Chinese Journal: Chinese Journal of Hematology Year: 2004 Type: Article