Clonal expansion of TCR Vbeta subfamily T cells induced by bcr3-abl2 peptide / 中华血液学杂志
Chinese Journal of Hematology
; (12): 95-99, 2004.
Article
in Zh
| WPRIM
| ID: wpr-291467
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clonal expansion of T cell receptor (TCR) Vbeta subfamily T cells from cord blood induced by bcr3-abl2 peptide in vitro.</p><p><b>METHODS</b>T cells from 3 units of cord blood were amplified by anti-CD(3) monoclonal antibody (McAb) and IL-2 with or without synthetic b3a2 peptide. T cell specific cytotoxicity was analyzed by lactate dehydrogenase (LDH) assay, TCR Vbeta subfamilies by using reverse transcriptase-polymerase chain reaction (RT-PCR) and genescan technique.</p><p><b>RESULTS</b>bcr3-abl2 peptide specific cytotoxicity T cells were successfully induced from the 3 units of cord blood by synthetic b3a2 peptide. Compared with that in CD(3) McAb induced cells, distribution pattern of TCR Vbeta repertoire was different in T cells induced with b3a2 peptide. Oligoclonal and oligoclonal tendency TCR Vbeta subfamily T cells could be identified in cord blood T cells induced by b3a2 peptide in 1 or 2 weeks, whereas those induced by anti-CD(3) McAb and IL-2 were mostly polyclonal.</p><p><b>CONCLUSION</b>The cytotoxicity T cells with anti-CML specificity could be induced by b3a2 peptide. The specific anti-CML cytotoxicity may be derived from the clonal expansion TCR Vbeta subfamily T cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
T-Lymphocytes, Cytotoxic
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Fusion Proteins, bcr-abl
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Interleukin-2
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CD3 Complex
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Genes, T-Cell Receptor beta
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Allergy and Immunology
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Antibodies, Monoclonal
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2004
Type:
Article