Molecular subtyping of Vibrio cholerae isolates by pulsed-field gel electrophoresis in Shenzhen from 1993 / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 491-494, 2007.
Article
in Zh
| WPRIM
| ID: wpr-294307
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the genetic relationships between different Vibrio cholerae isolates in Shenzhen from 1993 to 2002.</p><p><b>METHODS</b>Chromosomal DNA from 60 isolates was digested in seakem gold agrose with restriction enzyme Not I and plugs were then analyzed by pulsed-field gel electrophoresis. Pulsed-field gel electrophoresis (PFGE) patterns of V. cholerae isolates were clustered using BioNumerics software.</p><p><b>RESULTS</b>39 distinctive PFGE patterns were identified with each pattern having 20 to 30 bands. Most PFGE patterns were divided into cluster A or cluster B.</p><p><b>CONCLUSION</b>The closely related pandemic clone clusters of V. cholerae strains did exist in Shenzhen. PFGE of V. cholerae could be used for active surveillance and tracking for cholerae.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Vibrio cholerae
/
China
/
Cholera
/
Epidemiology
/
Classification
/
Electrophoresis, Gel, Pulsed-Field
/
Genetics
/
Methods
/
Microbiology
Limits:
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Epidemiology
Year:
2007
Type:
Article