Rapid identification of compounds in cortex moutan by liquid chromatography-tandem mass spectrometry / 药学学报

ABSTRACT

<p><b>AIM</b>To analyze the chemical constituents of cortex moutan by liquid chromatography coupled with electrospray ionization mass spectrometry.</p><p><b>METHODS</b>An on-line optimized HPLC-DAD/MS2 technique was employed.</p><p><b>RESULTS</b>In the negative ion detection mode, 38 components such as monoterpene glucosides, galloylglucoses and acetophenones were isolated. Among them, over thirty compounds were identified, including paeonol, paeonilflorin, oxypaeoniflorin, benzoylpaeoniflorin, benzoyloxypaeoniflorin, galloylpaeoniflorin, galloyloxypaeoniflorin, mundanpioside A, C, D, E, H, etc. by the high performance liquid chromatography with diode-array detection in parallel with electrospray ionization and quadrupole-time of flight tandem mass spectrometry (HPLC-DAD/ESI-MS2).</p><p><b>CONCLUSION</b>This method can be used to rapidly determine the constituents of cortex moutan.</p>