Prostate cancer cell vaccine transfected with 4-1BBL induces anti-tumor immunity in vitro / 中华男科学杂志
National Journal of Andrology
; (12): 773-777, 2010.
Article
in Zh
| WPRIM
| ID: wpr-295003
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the anti-tumor immunity in vitro induced by prostate cancer cell vaccine transfected with recombinant adenovirus encoding 4-1BBL in mice.</p><p><b>METHODS</b>The replication-deficient adenovirus AdEasy-1 system was used to construct recombinant adenovirus Ad-m4-1BBL and Ad-eGFP. The prostate cancer cell RM-1 of mice was transfected with Ad-m4-1BBL and Ad-eGFP, and treated with mitomycin (MMC) to produce TCV, TCV-Ad-eGFP and TCV-Ad-m4-1BBL, followed by co-culture with syngeneic murine spleen cells. Then the cytotoxic activity of the lymphocytes against RM-1 cells was analyzed with CCK-8 solution, and IL-2 and INF-gamma were detected by ELISA.</p><p><b>RESULTS</b>The 4-1BBL protein was highly expressed in the TCV-Ad-m4-1BBL of the 4-1BBL-transfected mice. TCV-Ad-m4-1BBL significantly increased the expressions of IL-2 ([180.24 +/- 2.22] pg/ml) and INF-gamma ([1512.46 +/- 23.64] pg/ml) as compared with TCV and TCV-Ad-eGFP (P < 0.05), and induced higher RM-1 cell specific cytotoxicity ([34.24 +/- 2.64]%) than the latter two ([9.82 +/- 1.48]%) and ([14.65 +/- 3. 21]%), (P < 0.05). But none of them exhibited significant cytotoxicity against hepatocellular carcinoma Hepal-6.</p><p><b>CONCLUSION</b>The m4-1BBL-expressing prostate cancer cell vaccine can effectively induce anti-tumor immune responses.</p>
Full text:
1
Index:
WPRIM
Main subject:
Prostatic Neoplasms
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Transfection
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Interleukin-2
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Coculture Techniques
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Cancer Vaccines
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Cytotoxicity, Immunologic
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Cell Line, Tumor
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Allergy and Immunology
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4-1BB Ligand
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Genetics
Limits:
Animals
Language:
Zh
Journal:
National Journal of Andrology
Year:
2010
Type:
Article