Effects of rat serum containing total flavonoid extract of Epimedium sagittatum on development of osteoblasts / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 928-931, 2008.
Article
in Zh
| WPRIM
| ID: wpr-295437
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of total flavonoid extract of Epimedium sagittatum (TFE) on the proliferation and differentiation of newborn rat calvarial osteoblasts (ROB).</p><p><b>METHOD</b>TFE was supplemented into the culture medium of ROB at 0. 1, 1, 10 and 100 microg x mL(-1) respectively. The serum of rats administered TFES (SRAT) was also added into the medium in a parallel treatment at 2.5%, 5% and 10% respectively. Their effects on cell proliferation and differentiation was studied by MTT and the analysis of osteogenic differentiation marks.</p><p><b>RESULT</b>TFE had no appreciable and on cell proliferation and differentiation at any concentration. However, 2.5% and 5% SRAT stimulated cell proliferation strongly and, 5% SRAT significantly promoted the maturation and function of osteoblast by improving the alkaline phosphatase activity, osteocalcin secretion, calcium deposition and the number of mineralized nodular structures.</p><p><b>CONCLUSION</b>The metabolites of TFE should be the anti-osteoporosis constitutes of Epimedium sagittatum.</p>
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
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Pharmacology
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Flavonoids
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Blood
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Calcification, Physiologic
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Drugs, Chinese Herbal
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Cell Differentiation
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Chemistry
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Calcium
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Rats, Wistar
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2008
Type:
Article