Identification of a novel PHOX2A gene mutation in a Chinese family with congenital fibrosis of extraocular muscles type 2 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 5-8, 2012.
Article
in Zh
| WPRIM
| ID: wpr-295545
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate potential mutation of PHOX2A (or ARIX) gene in a Chinese family affected with congenital fibrosis of extraocular muscles tyep 2 (CFEOM2).</p><p><b>METHODS</b>Genomic DNA was obtained from affected and unaffected members of the family. With an ABI PRSIM Linkage Mapping Set-MD10 kit, selected markers flanking the PHOX2A locus were used for linkage analysis. Exons of PHOX2 gene were amplified and sequenced. A total of 100 normal subjects were recruited as controls.</p><p><b>RESULTS</b>Genetic linkage was found at 11q13 between D11S4151 and D11S1320 and the PHOX2A gene. DNA sequencing has identified a heterozygous mutation in the exon 2 of the gene (227T to G, N76K). The same mutation was not found in the unaffected and 100 normal controls.</p><p><b>CONCLUSION</b>A mutation of the PHOX2A gene 227T to G is responsible for the onset of congenital fibrosis of extraocular muscles type 2 in this Chinese family.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pedigree
/
Congenital Abnormalities
/
Fibrosis
/
Molecular Sequence Data
/
Base Sequence
/
Case-Control Studies
/
Ocular Motility Disorders
/
China
/
Homeodomain Proteins
/
Genetics
Type of study:
Observational_studies
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2012
Type:
Article