Inclusion Bodies are Formed in SFTSV-infected Human Macrophages / 病毒学报
Chinese Journal of Virology
;
(6): 19-25, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-296222
ABSTRACT
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a new member in the genus Phlebovirus of the family Bunyaviridae identified in China. The SFTSV is also the causative pathogen of an emerging infectious disease severe fever with thrombocytopenia syndrome. Using immunofluorescent staining and confocal microscopy, the intracellular distribution of nucleocapsid protein (NP) in SFTSV-infected THP-1 cells was investigated with serial doses of SFTSV at different times after infection. Transmission electron microscopy was used to observe the ultrafine intracellular structure of SFTSV-infected THP-1 cells at different times after infection. SFTSV NP could form intracellular inclusion bodies in infected THP-1 cells. The association between NP-formed inclusion bodies and virus production was analyzed the size of the inclusion body formed 3 days after infection was correlated with the viral load in supernatants collected 7 days after infection. These findings suggest that the inclusion bodies formed in SFTSV-infected THP-1 cells could be where the SFTSV uses host-cell proteins and intracellular organelles to produce new viral particles.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Thrombocytopenia
/
Virology
/
Cell Line
/
China
/
Phlebovirus
/
Phlebotomus Fever
/
Genetics
/
Inclusion Bodies, Viral
/
Macrophages
Type of study:
Prognostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2016
Type:
Article
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