Scanning aneugen and clastogen by micronuclei analysis using flow cytometry / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 649-652, 2006.
Article
in Zh
| WPRIM
| ID: wpr-297615
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore a flow cytometry (FCM)-based method for discriminating aneugen- or clastogen-induced micronuclei.</p><p><b>METHODS</b>Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte (MN-RET) in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry.</p><p><b>RESULTS</b>The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse.</p><p><b>CONCLUSION</b>The flow cytometry-based micronucleus assay can be used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.</p>
Full text:
1
Index:
WPRIM
Main subject:
Reticulocytes
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Colchicine
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Cyclophosphamide
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Micronuclei, Chromosome-Defective
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Toxicity
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Flow Cytometry
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Methods
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Mutagens
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2006
Type:
Article