Studies on the apoptosis of RK13 cells induced by rabbit hemorrhagic disease virus / 病毒学报
Chinese Journal of Virology
;
(6): 316-317, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-297956
ABSTRACT
The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder, Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63% of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Virology
/
Cell Line
/
Cell Nucleus
/
Apoptosis
/
Hemorrhagic Disease Virus, Rabbit
/
Caliciviridae Infections
/
Caspase 3
/
DNA Fragmentation
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2009
Type:
Article
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