Apoptosis in nasopharyngeal carcinoma cell line NCE induced by curcumin and its molecular mechanism / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 612-616, 2006.
Article
in Zh
| WPRIM
| ID: wpr-298802
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism underlying the curcumin-induced apoptosis of nasopharyngeal carcinoma (NPC) cell line NCE cells.</p><p><b>METHODS</b>The characteristics of apoptosis were identified by observation acridine orange and ethidium bromide stains, ultrastructure assay, DNA fragmentation assay and TdT-mediated dUTP nick end labeling method (TUNEL). Mitochondrial membrane potential (delta psi m), activity of caspase-3, cytosol cytochrome C and expression of gene Fas were determined by flow cytometry (FCM), Western Blot and reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Several evidences of apoptosis were obtained from curcumin-treated NCE cells by acridine orange and ethidium bromide stains, ultrastructure identification, DNA fragmentation assay and TUNEL staining. And the mean TUNEL-positive rates increased significantly at the 3 different time points (12 h, 24 h and 48 h; 25.6%, 40.3% and 54.5%, respectively). In the curcumin-treated-groups, delta psi m altered significantly and the positive rates increased in a time-dependent manner. At the 3 different time points, the mean positive rates were 26.8%, 42.3% and 68.2%, respectively. When caspase-3 activity was detected, 80.5% cells presented proteases activities after 12 h incubation with curcumin. Western Blot analysis showed that cytoplasmic cytochrome C increased significantly after incubation with curcumin. Flow cytometry and RT-PCR analysis showed that curcumin could up-regulate the Fas expression in time-depended manner , the positive rates of Fas protein increased from 33.6% to 89.9%.</p><p><b>CONCLUSIONS</b>Curcumin induced apoptosis of NCE cells both through mitochondria-dependent pathway and death receptor pathway.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Nasopharyngeal Neoplasms
/
Apoptosis
/
Fas Receptor
/
Curcumin
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Cell Line, Tumor
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Caspase 3
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Membrane Potential, Mitochondrial
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Metabolism
Type of study:
Prognostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2006
Type:
Article